Gene editing analysis
Software and validation supporting an FDA-approved gene therapy
Simple. Reliable. Proven.
Citations
Users
Accuracy vs NGS
Measure gene-editing outcomes from a single sequencing reaction
TIDE (Tracking of Indels by DEcomposition) reads a standard Sanger trace from an edited cell population and reconstructs the spectrum of insertions and deletions produced by the edit. It returns editing efficiency and indel composition without worring about sequencing depth, library preparation or bioinformatics support.
What it measures
Overall editing efficiency, size and frequency of every indel present in the edited sample.
What it needs
A single Sanger sequencing reaction of a PCR amplicon and the guide RNA sequence.
Who uses it
1000s of R&D teams in academia, biotech and pharma; CDMOs and regulated manufacturing teams.
For the full mechanism behind the analysis, see our technology.
Trusted by researchers and manufacturing teams at
1000+ organisations worldwide
Try TIDE Right Now
Free to use app for non-profit organization, free to try for everyone else. Upload your Sanger trace files and get results in seconds. No installation needed.
Start TIDE — FreeWhat Sets TIDE Apart
A faster, simpler path from genome editing to answer — without sacrificing accuracy or compliance.
Fast and Simple
TIDE estimates your genome engineering success from a single Sanger sequencing reaction, and it is 99% as accurate as NGS. All you need is a PCR product.
Regulatory Compliance
Our GxP software is a 21 CFR Part 11 compliant alternative to NGS to help you de-risk any communication with the regulatory authority.
Scientific Excellence
With 2800 citations of the original paper and over 100,000 unique users on the public platform – TIDE is the most trusted editing analysis tool in the scientific community.
The Science Behind TIDE
Brinkman EK, Chen T, Amendola M, van Steensel B. Easy quantitative assessment of genome editing by sequence trace decomposition. Nucleic Acids Research, 42(22), e168 (2014).
Three Simple Steps
1. Edit Your Target
You can use any editing technology (CRISPR/Cas9, TALEN or ZFN) to modify your favorite target.
2. PCR Amplify and Sequence
Extract DNA from your population of edited organisms, PCR amplify the region you edited and perform capillary sequencing of the PCR product. Most providers have a one-day turnaround service.
3. Upload into TIDE and Analyze
Upload .ab1 trace files and guide RNA sequence into the TIDE web tool and hit analyze — no bioinformatics expertise required. Within seconds you get accurate editing efficiency estimates in addition to qualitative and quantitative information about the whole indel spectrum.
Production-ready Editing Efficiency Analysis
Powering World's First Approved CRISPR Therapy
Vertex Pharmaceuticals chose TIDE over NGS to evaluate the on-targeting editing frequency and potency of the one-time therapy for sickle cell disease and beta-thalassemia.
A Faster Path in Gene and Cell Therapy
NGS is a multistep, resource-intensive process unsuitable for multiple iterations and lot-release QC assays, whereas TIDE is a fast and reliable way to measure cell integrity and editing efficiency, delivering substantial time and money saving.
Edit or Check any DNA
TIDE is the tool of choice to simply, quickly and accurately assess DNA integrity, whether you edit the genome of mammalian cells, plant protoplasts or produce large batches of nucleic acids such as plasmids. We develop specific workflows to measure genomic contamination, copy number alteration, off-target detection and provide evidence at the DNA sequence level.
Regulation Compliance - Start early
TIDE GxP is more than a scientific tool. It is a regulation-compliant solution that simplifies critical assays and minimizes the risk that regulatory agencies will ask for proof of validation.
Built for Regulated Environments
We are the audit-ready provider of TIDE GxP, a software fit for mission-critical use in pharmaceutical development and production, in compliance with GxP and GAMP5 industry standards.
- Full validation documentation package
- IQ/OQ/PQ protocols and reports
- Risk-based approach per GAMP5
- 21 CFR Part 11 compliant audit trails
- Change control documentation
- Annual validation reviews
- Personnel training certificates
Community
Collaborate and get featured
TIDE is used daily by hundreds of researchers worldwide. We highlight real experiments and collaborations powered by TIDE — and we would like to feature yours. Tell us about your work and we may publish it for the entire TIDE community to see.
Start analysing your edits today
Join leading research institutions and pharmaceutical companies that are already using TIDE for genome-editing success.
No installation. No account required. Used in an FDA-approved gene therapy filing.